ADA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ADA.
Immunogen
Recombinant protein corresponding to amino acids of recombinant ADA.
Sequence
GDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:500-1:1000)
Western Blot(1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ADA polyclonal antibody (Cat # PAB28429) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with ADA polyclonal antibody (Cat # PAB28429) shows distinct cytoplasmic and nuclear positivity in germinal center cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of U-2 OS with ADA polyclonal antibody (Cat # PAB28429) at 1-4 ug/mL dilution shows positivity in plasma membrane & cytoplasm. -
Gene Info — ADA
Entrez GeneID
100Protein Accession#
P00813Gene Name
ADA
Gene Alias
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Gene Description
adenosine deaminase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq
Other Designations
adenosine aminohydrolase
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Interactome
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Pathway
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Disease
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