FLOT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant FLOT1.
Immunogen
Recombinant protein corresponding to human FLOT1.
Sequence
HQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQR
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (Human: 1:250-1:500, Rodent: 1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of lane 1: NIH-3T3 cell lysate and lane 2: NBT-II cell lysate using FLOT1 polyclonal antibody (Cat # PAB28424).Western Blot
Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: A-431, lane 4: Liver and lane 5: Tonsil using FLOT1 polyclonal antibody (Cat # PAB28424).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human stomach with FLOT1 polyclonal antibody (Cat # PAB28424) shows distinct cytoplasmic and membranous positivity with a granular pattern. -
Gene Info — FLOT1
Entrez GeneID
10211Protein Accession#
A2AB09Gene Name
FLOT1
Gene Alias
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Gene Description
flotillin 1
Omim ID
606998Gene Ontology
HyperlinkGene Summary
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000029208|integral membrane component of caveolae
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Interactome
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Pathway
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Disease
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