PNN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of lane 1: RT-4, lane 2: EFO-21 and lane 3: A-431 cell lysates using PNN polyclonal antibody (Cat # PAB28421).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with PNN polyclonal antibody (Cat # PAB28421) shows strong nuclear positivity in glandular cells.
Immunofluorescence
Immunofluorescence of A-431 cell line with PNN polyclonal antibody (Cat # PAB28421) shows positivity in nuclei but not nucleoli. Fixation/Permeabilization: PFA/Triton X-100
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PNN.
Immunogen
Recombinant protein corresponding to human PNN.
Sequence
EQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAEKE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:250-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of lane 1: RT-4, lane 2: EFO-21 and lane 3: A-431 cell lysates using PNN polyclonal antibody (Cat # PAB28421).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with PNN polyclonal antibody (Cat # PAB28421) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescence of A-431 cell line with PNN polyclonal antibody (Cat # PAB28421) shows positivity in nuclei but not nucleoli. Fixation/Permeabilization: PFA/Triton X-100 -
Gene Info — PNN
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Interactome
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Disease
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