STX5 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using STX5 polyclonal antibody (Cat # PAB28419).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human pancreas with STX5 polyclonal antibody (Cat # PAB28419) shows strong cytoplasmic positivity in exocrine glandular cells.
Immunofluorescence
Immunofluorescence of U-2 OS cell line with STX5 polyclonal antibody (Cat # PAB28419) shows positivity in nucleus but not nucleoli and golgi apparatus. Fixation/Permeabilization: PFA/Triton X-100
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant STX5.
Immunogen
Recombinant protein corresponding to human STX5.
Sequence
QIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using STX5 polyclonal antibody (Cat # PAB28419).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human pancreas with STX5 polyclonal antibody (Cat # PAB28419) shows strong cytoplasmic positivity in exocrine glandular cells.Immunofluorescence
Immunofluorescence of U-2 OS cell line with STX5 polyclonal antibody (Cat # PAB28419) shows positivity in nucleus but not nucleoli and golgi apparatus. Fixation/Permeabilization: PFA/Triton X-100 -
Gene Info — STX5
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Interactome
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Pathway
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Disease
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