Western Blot (1:100-1:250) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Immunofluorescence (1-4 ug/ml) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4 °C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with STX4 polyclonal antibody (Cat # PAB28414) shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunofluorescence of U-2 OS cell line with STX4 polyclonal antibody (Cat # PAB28414) shows positivity in plasma membrane. Fixation/Permeabilization: PFA/Triton X-100