SUB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of lane 1: NIH-3T3, lane 2: NBT-II and lane 3: PC12 cell lysates using SUB1 polyclonal antibody (Cat # PAB28411).
Western Blot
Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: A-431, lane 4: Liver and lane 5: Tonsil using SUB1 polyclonal antibody (Cat # PAB28411).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast with SUB1 polyclonal antibody (Cat # PAB28411) shows strong nuclear positivity in glandular cells.
Immunofluorescence
Immunofluorescence of U-2 OS cell line with SUB1 polyclonal antibody (Cat # PAB28411) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-100
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SUB1.
Immunogen
Recombinant protein corresponding to human SUB1.
Sequence
VSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQI
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of lane 1: NIH-3T3, lane 2: NBT-II and lane 3: PC12 cell lysates using SUB1 polyclonal antibody (Cat # PAB28411).Western Blot
Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: A-431, lane 4: Liver and lane 5: Tonsil using SUB1 polyclonal antibody (Cat # PAB28411).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast with SUB1 polyclonal antibody (Cat # PAB28411) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescence of U-2 OS cell line with SUB1 polyclonal antibody (Cat # PAB28411) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-100 -
Gene Info — SUB1
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