Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human heart muscle with MYH7 polyclonal antibody (Cat # PAB28402) shows strong cytoplasmic positivity in myocytes.
Immunofluorescence of U251 MG cell line with MYH7 polyclonal antibody (Cat # PAB28402) shows positivity in nucleus but not nucleoli, cytoplasm and focal adhesions. Fixation/Permeabilization: PFA/Triton X-100
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq
OTTHUMP00000027949,beta-myosin heavy chain,myhc-slow,myopathy, distal 1,myosin heavy chain (AA 1-96),myosin, heavy polypeptide 7, cardiac muscle, beta,rhabdomyosarcoma antigen MU-RMS-40.7A