PLA2G6 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using PLA2G6 polyclonal antibody (Cat # PAB28391).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine with PLA2G6 polyclonal antibody (Cat # PAB28391) shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescence of A-431 cell line with PLA2G6 polyclonal antibody (Cat # PAB28391) shows positivity in centrosome. Fixation/Permeabilization: PFA/Triton X-100
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PLA2G6.
Immunogen
Recombinant protein corresponding to human PLA2G6.
Sequence
QVLHTEVLQHLTDLIRNHPSWSVAHLAVELGIRECFHHSRIISCANCAENEEGCTPLHLACRKGDGEILVELVQYCHTQMDVTDYKGETVFHYAVQGDNSQVLQLLGRNAVAGLNQVNNQGLTPLHLACQLGKQEMVRVLLLCNARCNIMG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:250-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using PLA2G6 polyclonal antibody (Cat # PAB28391).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human small intestine with PLA2G6 polyclonal antibody (Cat # PAB28391) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescence of A-431 cell line with PLA2G6 polyclonal antibody (Cat # PAB28391) shows positivity in centrosome. Fixation/Permeabilization: PFA/Triton X-100 -
Gene Info — PLA2G6
Entrez GeneID
8398Protein Accession#
O60733Gene Name
PLA2G6
Gene Alias
CaI-PLA2, GVI, INAD1, IPLA2-VIA, PARK14, PLA2, PNPLA9, iPLA2
Gene Description
phospholipase A2, group VI (cytosolic, calcium-independent)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date. [provided by RefSeq
Other Designations
OTTHUMP00000028877|calcium-independent phospholipase A2|cytosolic, calcium-independent phospholipase A2|patatin-like phospholipase domain containing 9|phospholipase A2, group VI
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Interactome
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Pathway
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Disease
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