UBE2L3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant UBE2L3.
Immunogen
Recombinant protein corresponding to amino acids of recombinant UBE2L3.
Sequence
MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4 Lane 2: U-251 MG Lane 3: Human Plasma Lane 4: Liver Lane 5: Tonsil with UBE2L3 polyclonal antibody (Cat # PAB28318).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with UBE2L3 polyclonal antibody ( Cat # PAB28318 ) shows strong cytoplasmic positivity in subset of cells in seminiferus ducts. -
Gene Info — UBE2L3
Entrez GeneID
7332Protein Accession#
P68036Gene Name
UBE2L3
Gene Alias
E2-F1, L-UBC, UBCH7, UbcM4
Gene Description
ubiquitin-conjugating enzyme E2L 3
Omim ID
603721Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme UBCH7|ubiquitin-protein ligase
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Interactome
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Pathway
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Disease
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