HS3ST5 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with HS3ST5 polyclonal antibody (Cat # PAB28249).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with HS3ST5 polyclonal antibody (Cat # PAB28249) shows strong cytoplasmic positivity in Purkinje cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant HS3ST5.
Immunogen
Recombinant protein corresponding to amino acids of recombinant HS3ST5.
Sequence
DRLQPICPIEGRLGGARTQAEFPLRALQFKRGLLHEFRKGNASKEQVRLHDLVQQLPKAIIIGVRKGGTRALLEMLNLHPAVVKASQEIHF
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with HS3ST5 polyclonal antibody (Cat # PAB28249).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with HS3ST5 polyclonal antibody (Cat # PAB28249) shows strong cytoplasmic positivity in Purkinje cells. -
Gene Info — HS3ST5
Entrez GeneID
222537Gene Name
HS3ST5
Gene Alias
3-OST-5, 3OST5, HS3OST5
Gene Description
heparan sulfate (glucosamine) 3-O-sulfotransferase 5
Omim ID
609407Gene Ontology
HyperlinkGene Summary
HS3ST5 belongs to a group of heparan sulfate 3-O-sulfotransferases (EC 2.8.2.23) that transfer sulfate from 3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan sulfate and heparin (Mochizuki et al., 2003 [PubMed 12740361]).[supplied by OMIM
Other Designations
heparan sulfate 3-OST-5
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Pathway
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Disease
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