GPT polyclonal antibody
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Specification
Product Description
Sheep polyclonal antibody raised against native GPT (Pig).
Immunogen
Native purified GPT from pig heart.
Host
Sheep
Reactivity
Pig
Specificity
Cross reactivity against alanine transaminase from other sources is unknown.
Form
Lyophilized
Purification
This is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated in Storage Buffer.
Purity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
ELISA (1:5000 - 1:25000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPT
Entrez GeneID
2875Protein Accession#
P13191Gene Name
GPT
Gene Alias
AAT1, ALT1, GPT1
Gene Description
glutamic-pyruvate transaminase (alanine aminotransferase)
Omim ID
138200Gene Ontology
HyperlinkGene Summary
Glutamate-pyruvate transaminase (EC 2.6.1.2), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.[supplied by OMIM
Other Designations
alanine aminotransferase
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