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GPT polyclonal antibody 

  • Catalog # : PAB28097
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  • Specification
  • Product Description:
  • Sheep polyclonal antibody raised against native GPT (Pig).
  • Immunogen:
  • Native purified GPT from pig heart.
  • Host:
  • Sheep
  • Reactivity:
  • Pig
  • Specificity:
  • Cross reactivity against alanine transaminase from other sources is unknown.
  • Form:
  • Lyophilized
  • Purification:
  • This is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated in Storage Buffer.
  • Purity:
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody Reactive Against Native Purified Protein.
  • Recommend Usage:
  • ELISA (1:5000 - 1:25000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2875
  • Gene Name:
  • GPT
  • Gene Alias:
  • AAT1,ALT1,GPT1
  • Gene Description:
  • glutamic-pyruvate transaminase (alanine aminotransferase)
  • Gene Summary:
  • Glutamate-pyruvate transaminase (EC, also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.[supplied by OMIM
  • Other Designations:
  • alanine aminotransferase
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