ADA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of mouse pancreas, liver and Jurkat and Raji cells by ADA polyclonal antibody (Cat # PAB27925) at 1:5000. Lane 1: Reduced conditions(30 ul), Lane 2: purified ADA, Lane 3: Endogenous ADA in whole cell lysate from Jurkat and Raji cells (1:1 mixture) and Lane 4: Endogenous ADA in whole cell lysate from Mouse Pancreas and Liver (1:1 mixture).
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Specification
Product Description
Rabbit polyclonal antibody raised against native ADA (Bovine).
Immunogen
Native purified bovine ADA.
Host
Rabbit
Reactivity
Bovine
Form
Lyophilized
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
ELISA (1:5000 - 1:20000)
Immunoprecipitation (1:100)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of mouse pancreas, liver and Jurkat and Raji cells by ADA polyclonal antibody (Cat # PAB27925) at 1:5000. Lane 1: Reduced conditions(30 ul), Lane 2: purified ADA, Lane 3: Endogenous ADA in whole cell lysate from Jurkat and Raji cells (1:1 mixture) and Lane 4: Endogenous ADA in whole cell lysate from Mouse Pancreas and Liver (1:1 mixture).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADA
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