Choline Oxidase polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against native Choline Oxidase (Alcaligenes species).
Immunogen
Native purified Alcaligenes Choline Oxidase .
Host
Goat
Reactivity
Bacteria
Form
Lyophilized
Conjugation
Unconjugated
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
ELISA (1:5000 - 1:20000)
Immunoprecipitation (1:100)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis by Choline Oxidase polyclonal antibody (Cat # PAB27894) at 1:5000. Lane 1: Reduced Choline Oxidase at about 1ug (4% beta-Mercaptoethanol, boiled for 5 minutes). Lane 2: Non-reduced Choline Oxidase at 0.25 ug.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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