ECSIT polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant ECSIT.
Immunogen
Recombinant protein corresponding to amino acids of human ECSIT
Sequence
HMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPM
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200 - 1:500)
Western Blot (1:100 - 1:250)
Immunoflurorescence (1-4 µg/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4 Lane 2: U-251 MG Lane 3: Human Plasma Lane 4: Liver Lane 5: Tonsil with ECSIT polyclonal antibody ( Cat # PAB28035 ) at 1:100 - 1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with ECSIT polyclonal antibody ( Cat # PAB28035 ) shows strong cytoplasmic positivity in glandular cells at 1:200 - 1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with ECSIT polyclonal antibody ( Cat # PAB28035 ) at 1-4 ug/ml dilution shows positivity in nucleus but not nucleoli & cytoplasm. -
Gene Info — ECSIT
Entrez GeneID
51295Gene Name
ECSIT
Gene Alias
SITPEC
Gene Description
ECSIT homolog (Drosophila)
Omim ID
608388Gene Ontology
HyperlinkOther Designations
evolutionarily conserved signaling intermediate in Toll pathway|likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved|signaling intermediate in Toll pathway evolutionarily conserved ortholog|signaling intermediate in Tol
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Interactomes
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Pathways
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