SPHK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SPHK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SPHK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
The SPHK2 polyclonal antibody (Cat # PAB2802) is used in Western blot (Lane 1) to detect c-myc-tagged SPHK2 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 2) . Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SPHK2 polyclonal antibody (Cat # PAB2802) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — SPHK2
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Interactome
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Pathway
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Publication Reference
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Sphingosine 1-phosphate modulates spinal nociceptive processing.
Coste O, Brenneis C, Linke B, Pierre S, Maeurer C, Becker W, Schmidt H, Gao W, Geisslinger G, Scholich K.
The Journal of Biological Chemistry 2008 Sep; 283(47):32442.
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Sphingosine 1-phosphate modulates spinal nociceptive processing.
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