The SPHK2 polyclonal antibody (Cat # PAB2802) is used in Western blot (Lane 1) to detect c-myc-tagged SPHK2 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 2) . Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH) .
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SPHK2 polyclonal antibody (Cat # PAB2802) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.