SPHK1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
(LEFT) The SPHK1 polyclonal antibody (Cat # PAB2800) is used in Western blot (Lane 2) to detect c-myc-tagged SPHK1 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). (RIGHT) Western blot analysis of SPHK1 polyclonal antibody (Cat # PAB2800) in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1 (arrow) was detected using the purified polyclonal antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SPHK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SPHK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
(LEFT) The SPHK1 polyclonal antibody (Cat # PAB2800) is used in Western blot (Lane 2) to detect c-myc-tagged SPHK1 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). (RIGHT) Western blot analysis of SPHK1 polyclonal antibody (Cat # PAB2800) in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1 (arrow) was detected using the purified polyclonal antibody. -
Gene Info — SPHK1
Entrez GeneID
8877Protein Accession#
Q9NYA1Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K
Nature Genetics 2003 Dec; 36(1):40.
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Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution.
Melendez AJ, Carlos-Dias E, Gosink M, Allen JM, Takacs L.
Gene 2000 Jun; 251(1):19.
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Functional characterization of human sphingosine kinase-1.
Nava VE, Lacana E, Poulton S, Liu H, Sugiura M, Kono K, Milstien S, Kohama T, Spiegel S.
FEBS Letters 2000 May; 473(1):81.
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
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