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(LEFT) The SPHK1 polyclonal antibody (Cat # PAB2800) is used in Western blot (Lane 2) to detect c-myc-tagged SPHK1 in transfected 293 cell lysate (ac-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH). (RIGHT) Western blot analysis of SPHK1 polyclonal antibody (Cat # PAB2800) in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1 (arrow) was detected using the purified polyclonal antibody.
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM