DENND1C polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DENND1C polyclonal antibody (Cat # PAB27942) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with DENND1C polyclonal antibody (Cat # PAB27942) shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells at 1:2500-1:5000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with DENND1C polyclonal antibody (Cat # PAB27942) at 1-4 ug/mL dilution shows positivity in plasma membrane and focal adhesions.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DENND1C.
Immunogen
Recombinant protein corresponding to amino acids of human DENND1C.
Sequence
PLSPEDEGCPWAEEALDSSFLGSGEELDLLSEILDSLSMGAKSAGSLRPSQSLDCCHRGDLDSCFSLPNIPRWQPDDKKL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:2500-1:5000)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DENND1C polyclonal antibody (Cat # PAB27942) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with DENND1C polyclonal antibody (Cat # PAB27942) shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells at 1:2500-1:5000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with DENND1C polyclonal antibody (Cat # PAB27942) at 1-4 ug/mL dilution shows positivity in plasma membrane and focal adhesions. -
Gene Info — DENND1C
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