DUS2L polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DUS2L.
Immunogen
Recombinant protein corresponding to amino acids of human DUS2L.
Sequence
SLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGT
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DUS2L polyclonal antibody (Cat # PAB27928) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with DUS2L polyclonal antibody (Cat # PAB27928) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DUS2L polyclonal antibody (Cat # PAB27928) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — DUS2L
Entrez GeneID
54920Gene Name
DUS2L
Gene Alias
DUS2, FLJ20399, SMM1, URLC8
Gene Description
dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
Omim ID
609707Gene Ontology
HyperlinkGene Summary
Dihydrouridine synthase catalyzes reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al., 2005 [PubMed 15994936]).[supplied by OMIM
Other Designations
dihydrouridine synthase 2-like, SMM1 homolog|up-regulated in lung cancer 8
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