ANKRD2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412545) with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with ANKRD2 polyclonal antibody (Cat # PAB27887) shows strong cytoplasmic positivity in myocytes at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1-4 ug/mL dilution shows positivity in vesicles.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ANKRD2.
Immunogen
Recombinant protein corresponding to amino acids of human ANKRD2.
Sequence
IIKLLLLHGADMMTKNLAGKTPTDLVQLWQADTRHALEHPEPGAEHNGLEGPNDSGRETPQPVPAQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412545) with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with ANKRD2 polyclonal antibody (Cat # PAB27887) shows strong cytoplasmic positivity in myocytes at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1-4 ug/mL dilution shows positivity in vesicles. -
Gene Info — ANKRD2
Entrez GeneID
26287Gene Name
ANKRD2
Gene Alias
ARPP, MGC104314
Gene Description
ankyrin repeat domain 2 (stretch responsive muscle)
Omim ID
610734Gene Ontology
HyperlinkGene Summary
ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy (Miller et al., 2003 [PubMed 14583192]).[supplied by OMIM
Other Designations
OTTHUMP00000059423|ankyrin repeat domain 2|ankyrin-repeat protein
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Interactome
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Disease
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