BCL2L14 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408531) with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with BCL2L14 polyclonal antibody (Cat # PAB27877) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1-4 ug/mL dilution shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant BCL2L14.
Immunogen
Recombinant protein corresponding to amino acids of human BCL2L14.
Sequence
GQRTLEYQDSHSQQWSRCLSNVEQCLEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGFKSKEIFVTEGLSFQLQGHVPVASSSKKDEEEQILAKIVELL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408531) with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with BCL2L14 polyclonal antibody (Cat # PAB27877) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with BCL2L14 polyclonal antibody (Cat # PAB27877) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — BCL2L14
Entrez GeneID
79370Gene Name
BCL2L14
Gene Alias
BCLG
Gene Description
BCL2-like 14 (apoptosis facilitator)
Omim ID
606126Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported for this gene. [provided by RefSeq
Other Designations
BCL2-like 14|apoptosis regulator BCL-G
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Interactome
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Disease
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