NOP2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NOP2.
Immunogen
Recombinant protein corresponding to amino acids of human NOP2.
Sequence
KLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVPIGATPEYLAGH
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOP2 polyclonal antibody (Cat # PAB27870) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skin with NOP2 polyclonal antibody (Cat # PAB27870) shows distinct nucleolar positivity in keratinocytes at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with NOP2 polyclonal antibody (Cat # PAB27870) at 1-4 ug/mL dilution shows positivity in nucleoli. -
Gene Info — NOP2
Entrez GeneID
4839Gene Name
NOP2
Gene Alias
MGC117384, MGC149287, MGC149288, NOL1, NOP120, NSUN1, p120
Gene Description
NOP2 nucleolar protein homolog (yeast)
Omim ID
164031Gene Ontology
HyperlinkGene Summary
member 1|nucleolar protein 1
Other Designations
NOL1/NOP2/Sun domain family, member 1|nucleolar protein 1, 120kDa|nucleolar protein 2 homolog
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