MAPK12 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAPK12.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MAPK12.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse lung tissue lysate (35 ug/lane) with MAPK12 polyclonal antibody (Cat # PAB2786).Western Blot (Transfected lysate)
Western blot analysis of MAPK12 (arrow) using MAPK12 polyclonal antibody (Cat # PAB2786).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK12 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAPK12 polyclonal antibody (Cat # PAB2786) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — MAPK12
Entrez GeneID
6300Protein Accession#
P53778Gene Name
MAPK12
Gene Alias
ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3
Gene Description
mitogen-activated protein kinase 12
Omim ID
602399Gene Ontology
HyperlinkGene Summary
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq
Other Designations
mitogen-activated protein kinase 3|stress-activated protein kinase 3
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Interactome
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Pathway
- Amyotrophic lateral sclerosis (ALS)
- Epithelial cell signaling in Helicobacter pylori infection
- Fc epsilon RI signaling pathway
- GnRH signaling pathway
- Leukocyte transendothelial migration
- MAPK signaling pathway
- Neurotrophin signaling pathway
- T cell receptor signaling pathway
- Toll-like receptor signaling pathway
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Disease
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Publication Reference
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Nuclear export of ERK3 by a CRM1-dependent mechanism regulates its inhibitory action on cell cycle progression.
Julien C, Coulombe P, Meloche S.
The Journal of Biological Chemistry 2003 Oct; 278(43):42615.
Application:IF, Mouse, NIH 3T3 fibroblasts.
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Different domains of the mitogen-activated protein kinases ERK3 and ERK2 direct subcellular localization and upstream specificity in vivo.
Robinson MJ, Xu Be BE, Stippec S, Cobb MH.
The Journal of Biological Chemistry 2002 Feb; 277(7):5094.
Application:KA, WB-Re, Recombinant protein.
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Nuclear export of ERK3 by a CRM1-dependent mechanism regulates its inhibitory action on cell cycle progression.
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