MAP3K12 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP3K12.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP3K12.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of MAP3K12 polyclonal antibody (Cat # PAB2777) in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B) . MAP3K12 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP3K12 polyclonal antibody (Cat # PAB2777) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — MAP3K12
Entrez GeneID
7786Protein Accession#
NP_006292;Q12852Gene Name
MAP3K12
Gene Alias
DLK, MUK, ZPK, ZPKP1
Gene Description
mitogen-activated protein kinase kinase kinase 12
Omim ID
600447Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. [provided by RefSeq
Other Designations
dual leucine zipper kinase DLK|leucine zipper protein kinase|protein kinase MUK|zipper protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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SP3 acts as a positive regulator on the core promoter of human ZPK gene.
Itoh A, Wang Z, Ito Y, Reddy UR, Itoh T.
Biochemical and Biophysical Research Communications 2004 Jan; 313(3):612.
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MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase.
Fukuyama K, Yoshida M, Yamashita A, Deyama T, Baba M, Suzuki A, Mohri H, Ikezawa Z, Nakajima H, Hirai S, Ohno S.
The Journal of Biological Chemistry 2000 Jul; 275(28):21247.
Application:IF, Monkey, COS-1 cells.
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ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation.
Reddy UR, Basu A, Bannerman P, Ikegaki N, Reddy CD, Pleasure D.
Oncogene 1999 Aug; 18(31):4474.
Application:Func, IF, IHC, KA, WB-Ce, WB-Re, Mouse, NIH/3T3, NT2 cells, Recombinant protein.
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SP3 acts as a positive regulator on the core promoter of human ZPK gene.
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