CAMK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CAMK1 (arrow) using CAMK1 polyclonal antibody (Cat # PAB2769). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2) (Origene Technologies).
Western Blot
The CAMK1 polyclonal antibody (Cat # PAB2769) is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CAMK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CAMK1.
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CAMK1 (arrow) using CAMK1 polyclonal antibody (Cat # PAB2769). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1 gene (Lane 2) (Origene Technologies).Western Blot
The CAMK1 polyclonal antibody (Cat # PAB2769) is used in Western blot to detect CAMK1 in HeLa cell lysate (lane 1) and primate brain tissue lysate (lane 2) . -
Gene Info — CAMK1
Entrez GeneID
8536Protein Accession#
Q14012Gene Name
CAMK1
Gene Alias
CAMKI, MGC120317, MGC120318
Gene Description
calcium/calmodulin-dependent protein kinase I
Omim ID
604998Gene Ontology
HyperlinkGene Summary
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq
Other Designations
OTTHUMP00000158527
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Interactome
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Disease
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Publication Reference
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Neuronal Pentraxin 2 is a regulator of clear cell renal cell carcinoma malignancy through activation of the AMPA-selective glutamate receptor 4.
Von Roemeling CA, Radisky DC, Marlow LA, Cooper SJ, Grebe SK, Anastasiadis PZ, Tun HW, Copland JA.
Cancer Research 2014 Sep; 74(17):4796.
Application：WB-Ce, Human, Caki1, THJ16T, HeLa cells.
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Calmodulin-dependent kinase I regulates adrenal cell expression of aldosterone synthase.
Condon JC, Pezzi V, Drummond BM, Yin S, Rainey WE.
Endocrinology 2002 Sep; 143(9):3651.
Application：IHC-P, WB, Human, H295R cells, Human adrenal cortex, brain .
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Characterization of substrate phosphorylation and use of calmodulin mutants to address implications from the enzyme crystal structure of calmodulin-dependent protein kinase I.
Chin D, Winkler KE, Means AR.
The Journal of Biological Chemistry 1997 Dec; 272(50):31235.
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Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase.
Haribabu B, Hook SS, Selbert MA, Goldstein EG, Tomhave ED, Edelman AM, Snyderman R, Means AR.
The EMBO Journal 1995 Aug; 14(15):3679.
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Neuronal Pentraxin 2 is a regulator of clear cell renal cell carcinoma malignancy through activation of the AMPA-selective glutamate receptor 4.
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