Znrf1 polyclonal antibody
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More Files
- More Functions
Western Blot
Lane 1: N2a mock transfection.
Lane 2: N2a overexpressing mouse Znrf1 and probed with Znrf1 polycolnal antibody (Cat # PAB27672) (1ug/ml).
Lane 3: Znrf1 polycolnal antibody (Cat # PAB27672) staining of mouse brain lysates and Lane 4: adult cerebellum. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Znrf1 polycolnal antibody (Cat # PAB27672) (10ug/ml) staining of paraffin embedded mouse cerebral cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Znrf1.
Immunogen
A synthetic peptide corresponding to internal region of mouse Znrf1.
Sequence
SDSTYAHGNGYQET
Host
Goat
Theoretical MW (kDa)
28
Reactivity
Chicken, Dog, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3ug/ml)
Immunohistochemistry (10ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Lane 1: N2a mock transfection.
Lane 2: N2a overexpressing mouse Znrf1 and probed with Znrf1 polycolnal antibody (Cat # PAB27672) (1ug/ml).
Lane 3: Znrf1 polycolnal antibody (Cat # PAB27672) staining of mouse brain lysates and Lane 4: adult cerebellum. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Znrf1 polycolnal antibody (Cat # PAB27672) (10ug/ml) staining of paraffin embedded mouse cerebral cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100). -
Gene Info — Znrf1
Entrez GeneID
170737Protein Accession#
NP_573469.1;NP_001162093.1Gene Name
Znrf1
Gene Alias
B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283
Gene Description
zinc and ring finger 1
Gene Ontology
HyperlinkOther Designations
peripheral nerve injury protein nin283|ring finger protein 42|zinc ring finger protein 1
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