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Last updated: 2016/12/4

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Znrf1 polyclonal antibody

  • Catalog # : PAB27672
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of Znrf1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of mouse Znrf1.
  • Sequence:
  • SDSTYAHGNGYQET
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 28
  • Reactivity:
  • Chicken, Dog, Human, Mouse, Pig, Rat
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-3ug/ml)
    Immunohistochemistry (10ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Lane 1: N2a mock transfection.
    Lane 2: N2a overexpressing mouse Znrf1 and probed with Znrf1 polycolnal antibody (Cat # PAB27672) (1ug/ml).
    Lane 3: Znrf1 polycolnal antibody (Cat # PAB27672) staining of mouse brain lysates and Lane 4: adult cerebellum. Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Znrf1 polycolnal antibody (Cat # PAB27672) (10ug/ml) staining of paraffin embedded mouse cerebral cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_573469.1;NP_001162093.1
  • Gene Name:
  • Znrf1
  • Gene Alias:
  • B830022L21Rik,MGC101991,Rnf42,Zrfp1,nin283
  • Gene Description:
  • zinc and ring finger 1
  • Other Designations:
  • peripheral nerve injury protein nin283,ring finger protein 42,zinc ring finger protein 1
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