MYOT polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MYOT polycolnal antibody (Cat # PAB27663) (0.1ug/ml) staining of human skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MYOT.
Immunogen
A synthetic peptide corresponding to amino acids 209-221 at internal region of human MYOT.
Sequence
DSQQHNSEHARLQ
Host
Goat
Theoretical MW (kDa)
60
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MYOT polycolnal antibody (Cat # PAB27663) (0.1ug/ml) staining of human skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — MYOT
Entrez GeneID
9499Protein Accession#
NP_006781.1;NP_001129412.1Gene Name
MYOT
Gene Alias
LGMD1, LGMD1A, TTID
Gene Description
myotilin
Gene Ontology
HyperlinkGene Summary
This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined
Other Designations
titin immunoglobulin domain protein (myotilin)
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Interactome
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Disease
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