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Last updated: 2017/4/23

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MYOT polyclonal antibody

  • Catalog # : PAB27663
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of MYOT.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 209-221 at internal region of human MYOT.
  • Sequence:
  • DSQQHNSEHARLQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 60
  • Reactivity:
  • Human
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Western Blot (0.1-0.3ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • MYOT polycolnal antibody (Cat # PAB27663) (0.1ug/ml) staining of human skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9499
  • Protein Accession#:
  • NP_006781.1;NP_001129412.1
  • Gene Name:
  • MYOT
  • Gene Alias:
  • LGMD1,LGMD1A,TTID
  • Gene Description:
  • myotilin
  • Gene Summary:
  • This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined
  • Other Designations:
  • titin immunoglobulin domain protein (myotilin)
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