PLIN3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PLIN3.
Immunogen
A synthetic peptide corresponding to amino acids 154-167 at internal region of human PLIN3.
Sequence
TRGAVQSGVDKTKS
Host
Goat
Theoretical MW (kDa)
48
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
PLIN3 polycolnal antibody (Cat # PAB27662) (0.03ug/ml) staining of Lane 1: HeLa and Lane 2: human colon cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — PLIN3
Entrez GeneID
10226Protein Accession#
NP_005808.3;NP_001157661.1;NP_001157666.1Gene Name
PLIN3
Gene Alias
MGC11117, MGC2012, PP17, TIP47, M6PRBP1
Gene Description
mannose-6-phosphate receptor binding protein 1
Omim ID
602702Gene Ontology
HyperlinkGene Summary
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
MPR-BINDING PROTEIN, 47-KD|MPR-binding protein 47 kDa|cargo selection protein (mannose 6 phosphate receptor binding protein)|cargo selection protein TIP47|mannose 6 phosphate receptor binding protein 1|placental protein 17|tail-interacting protein, 47 kD
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Interactome
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