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Last updated: 2016/11/27

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AP2A1 polyclonal antibody

  • Catalog # : PAB27659
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of AP2A1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 706-727 at internal region of human AP2A1.
  • Sequence:
  • ELEPPAPESPMALLADPAPAAD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 110
  • Reactivity:
  • Chicken, Human, Mouse, Pig, Rat
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Western Blot (0.01-0.03ug/ml)
    Immunocytochemistry
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of mouse brain lysate (~5ug protein in SDSPAGE buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • AP2A1 polycolnal antibody (Cat # PAB27659) (0.01ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunocytochemistry
  • Immunocytochemistry
  • AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 160
  • Gene Name:
  • AP2A1
  • Gene Alias:
  • ADTAA,AP2-ALPHA,CLAPA1
  • Gene Description:
  • adaptor-related protein complex 2, alpha 1 subunit
  • Gene Summary:
  • This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
  • Other Designations:
  • 100 kDa coated vesicle protein A,adaptin, alpha A,clathrin-associated/assembly/adaptor protein, large, alpha 1
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