AP2A1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of mouse brain lysate (~5ug protein in SDSPAGE buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Tissue lysate)
AP2A1 polycolnal antibody (Cat # PAB27659) (0.01ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry
AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AP2A1.
Immunogen
A synthetic peptide corresponding to amino acids 706-727 at internal region of human AP2A1.
Sequence
ELEPPAPESPMALLADPAPAAD
Host
Goat
Theoretical MW (kDa)
110
Reactivity
Chicken, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.01-0.03ug/ml)
Immunocytochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of mouse brain lysate (~5ug protein in SDSPAGE buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Tissue lysate)
AP2A1 polycolnal antibody (Cat # PAB27659) (0.01ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry
AP2A1 polycolnal antibody (Cat # PAB27659) (0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK. -
Gene Info — AP2A1
Entrez GeneID
160Protein Accession#
NP_055018.2Gene Name
AP2A1
Gene Alias
ADTAA, AP2-ALPHA, CLAPA1
Gene Description
adaptor-related protein complex 2, alpha 1 subunit
Omim ID
601026Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
Other Designations
100 kDa coated vesicle protein A|adaptin, alpha A|clathrin-associated/assembly/adaptor protein, large, alpha 1
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Interactome
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