EEF1A2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
EEF1A2 polycolnal antibody (Cat # PAB27657) (0.5ug/ml) staining of Lane 1: mouse skeletal muscle, Lane 2: rat skeletal muscle, Lane 3: mouse heart and Lane 4: rat heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Cell lysate)
EEF1A2 polycolnal antibody (Cat # PAB27657) (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EEF1A2.
Immunogen
A synthetic peptide corresponding to internal region of human EEF1A2.
Sequence
NPATVPFVPIS
Host
Goat
Theoretical MW (kDa)
50
Reactivity
Chicken, Dog, Human, Mouse, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-2ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EEF1A2 polycolnal antibody (Cat # PAB27657) (0.5ug/ml) staining of Lane 1: mouse skeletal muscle, Lane 2: rat skeletal muscle, Lane 3: mouse heart and Lane 4: rat heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Cell lysate)
EEF1A2 polycolnal antibody (Cat # PAB27657) (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — EEF1A2
Entrez GeneID
1917Protein Accession#
NP_001949.1Gene Name
EEF1A2
Gene Alias
EEF1AL, EF-1-alpha-2, EF1A, FLJ41696, HS1, STN, STNL
Gene Description
eukaryotic translation elongation factor 1 alpha 2
Omim ID
602959Gene Ontology
HyperlinkGene Summary
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000031776|elongation factor 1-alpha 2|elongation factor-1 alpha|statin S1|statin-like
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