HSP31 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
HSP31 polycolnal antibody (Cat # PAB27652) (0.05ug/ml) staining of yeast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HSP31.
Immunogen
A synthetic peptide corresponding to amino acids 50-64 at internal region of yeast HSP31.
Sequence
ETGKFGWDEHSLAKD
Host
Goat
Theoretical MW (kDa)
24
Reactivity
Yeast
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.02-0.06ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
HSP31 polycolnal antibody (Cat # PAB27652) (0.05ug/ml) staining of yeast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — HSP31
Entrez GeneID
852146Protein Accession#
NP_010822.1Gene Name
HSP31
Gene Alias
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Gene Description
Hsp31p
Gene Ontology
HyperlinkOther Designations
Possible chaperone and cysteine protease with similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site
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