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Last updated: 2022/5/22

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C5AR1 polyclonal antibody 

  • Catalog # : PAB27648
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against peptide with C5AR1.
  • Immunogen:
  • A synthetic peptide corresponding to C-RESKSFTRSTVDT, from the C Terminus of the protein sequence according to NP_001727.1.
  • Sequence:
  • RESKSFTRSTVDT
  • Host:
  • Goat
  • Reactivity:
  • Human
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (6 - 8 ug/mL)
    Flow Cytometry (10 ug/mL)
    Western Blot (0.01 - 0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • C5AR1 polyclonal antibody (Cat # PAB27648) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • C5AR1 polyclonal antibody (Cat # PAB27648) (6 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
  • Immunofluorescence
  • Immunofluorescence
  • C5AR1 polyclonal antibody (Cat # PAB27648) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
  • Flow Cytometry
  • Flow Cytometry
  • C5AR1 polyclonal antibody (Cat # PAB27648) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 728
  • Gene Name:
  • C5AR1
  • Gene Alias:
  • C5A,C5AR,C5R1,CD88
  • Gene Description:
  • complement component 5a receptor 1
  • Other Designations:
  • C5a anaphylatoxin receptor,C5a ligand,complement component 5 receptor 1
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