SLC1A3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SLC1A3 polycolnal antibody (Cat # PAB27643) (0.1ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SLC1A3.
Immunogen
A synthetic peptide corresponding to amino acids 197-208 at internal region of human SLC1A3.
Sequence
RSFKVPIQANET
Host
Goat
Theoretical MW (kDa)
60
Reactivity
Human, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SLC1A3 polycolnal antibody (Cat # PAB27643) (0.1ug/ml) staining of human frontal cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — SLC1A3
Entrez GeneID
6507Protein Accession#
NP_004163.3;NP_001160167.1Gene Name
SLC1A3
Gene Alias
EA6, EAAT1, FLJ25094, GLAST, GLAST1
Gene Description
solute carrier family 1 (glial high affinity glutamate transporter), member 3
Omim ID
600111Gene Ontology
HyperlinkGene Summary
Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system (Kirschner et al., 1994 [PubMed 8001975]).[supplied by OMIM
Other Designations
glutamate transporter variant EAAT1ex9skip
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Interactome
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Disease
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