Ncf1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Ncf1.
Immunogen
A synthetic peptide corresponding to internal region of mouse Ncf1.
Sequence
DEAEDPDPNYAGEP
Host
Goat
Theoretical MW (kDa)
45
Reactivity
Chicken, Dog, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (0.03-0.1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Ncf1 polycolnal antibody (Cat # PAB27626) (0.03ug/ml) staining of mouse thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — Ncf1
Entrez GeneID
17969Protein Accession#
NP_035006.3Gene Name
Ncf1
Gene Alias
NOXO2, Ncf-1, p47<phox>, p47phox
Gene Description
neutrophil cytosolic factor 1
Gene Ontology
HyperlinkOther Designations
NADPH oxidase subunit (47 kD)|NADPH oxidase subunit (47kDa)|OTTMUSP00000022112
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