PSMB4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PSMB4 polycolnal antibody (Cat # PAB27598) (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PSMB4.
Immunogen
A synthetic peptide corresponding to internal region of human PSMB4.
Sequence
QIATVTEKGVE
Host
Goat
Theoretical MW (kDa)
25
Reactivity
Chicken, Dog, Human, Mouse, Rabbit, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PSMB4 polycolnal antibody (Cat # PAB27598) (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — PSMB4
Entrez GeneID
5692Protein Accession#
NP_002787.2Gene Name
PSMB4
Gene Alias
HN3, HsN3, PROS26
Gene Description
proteasome (prosome, macropain) subunit, beta type, 4
Omim ID
602177Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq
Other Designations
macropain beta chain|multicatalytic endopeptidase complex beta chain|proteasome beta 4 subunit|proteasome beta chain|proteasome chain 3|proteasome subunit HsN3|proteasome subunit, beta type, 4
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Interactome
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Pathway
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Disease
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