DAPP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10ug protein in RIPA buffer) over expressing human DAPP1 with DYKDDDDK tag probed with DAPP1 polycolnal antibody (Cat # PAB27587) (0.1ug/ml) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in Lane 3.
Lane 2: Mock-transfected HEK293 probed with DAPP1 polycolnal antibody (Cat # PAB27587) (1mg/ml). Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DAPP1.
Immunogen
A synthetic peptide corresponding to near C-terminus region of human DAPP1.
Sequence
KLSQIRKQLNQGE
Host
Goat
Theoretical MW (kDa)
35
Reactivity
Chicken, Dog, Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.1-0.3ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10ug protein in RIPA buffer) over expressing human DAPP1 with DYKDDDDK tag probed with DAPP1 polycolnal antibody (Cat # PAB27587) (0.1ug/ml) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in Lane 3.
Lane 2: Mock-transfected HEK293 probed with DAPP1 polycolnal antibody (Cat # PAB27587) (1mg/ml). Primary incubations were for 1 hour. Detected by chemiluminescence. -
Gene Info — DAPP1
Entrez GeneID
27071Protein Accession#
NP_055210.2Gene Name
DAPP1
Gene Alias
BAM32, DKFZp667E0716
Gene Description
dual adaptor of phosphotyrosine and 3-phosphoinositides
Omim ID
605768Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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