HEK293 lysate (10ug protein in RIPA buffer) over expressing human CMA1 with DYKDDDDK tag probed with CMA1 polycolnal antibody (Cat # PAB27585) (0.5ug/ml) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in Lane 3. Lane 2: Mock-transfected HEK293 probed with CMA1 polycolnal antibody (Cat # PAB27585) (1mg/ml). Primary incubations were for 1 hour. Detected by chemiluminescence.
This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq