PCOLCE polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PCOLCE.
Immunogen
A synthetic peptide corresponding to C-terminus of human PCOLCE.
Sequence
QILTNLSKRKCPSQP
Host
Goat
Theoretical MW (kDa)
55
Reactivity
Dog, Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.3-1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PCOLCE polycolnal antibody (Cat # PAB27535) (0.3ug/ml) staining of human seart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — PCOLCE
Entrez GeneID
5118Protein Accession#
NP_002584.2Gene Name
PCOLCE
Gene Alias
PCPE, PCPE1
Gene Description
procollagen C-endopeptidase enhancer
Omim ID
600270Gene Ontology
HyperlinkGene Summary
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq
Other Designations
procollagen C-proteinase enhancer 1|procollagen, type 1, COOH-terminal proteinase enhancer
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