COPA polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of COPA.
Immunogen
A synthetic peptide corresponding to internal region of human COPA.
Sequence
CTRASNLENST
Host
Goat
Theoretical MW (kDa)
140
Reactivity
Chicken, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:1000)
Western Blot (0.5-2ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
COPA polycolnal antibody (Cat # PAB27525) (0.5ug/ml) staining of human prostate lysate (35ug protein in RIPA buffer) with (Lane: 2) and without (Lane 1) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — COPA
Entrez GeneID
1314Protein Accession#
NP_001091868.1;NP_004362.2Gene Name
COPA
Gene Alias
FLJ26320, HEP-COP
Gene Description
coatomer protein complex, subunit alpha
Omim ID
601924Gene Ontology
HyperlinkGene Summary
In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000031846|OTTHUMP00000031847|alpha coat protein|xenin
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