NSUN7 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant NSUN7.
Immunogen
Recombinant protein corresponding to amino acids of human NSUN7.
Sequence
SEVQEVENLLNSFKIKLAAALARCRIKHDALSIYHILPETVRKQELRASTLPLYAWINTCKISPEEVYNNLKRRGYNKVKSVLHIDDKVFAVDQHCYDVLIFPSHLKNDLINIDLFKDYKLIFQDKSRSLA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NSUN7 polyclonal antibody (Cat # PAB27477) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with NSUN7 polyclonal antibody (Cat # PAB27477) shows moderate cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with NSUN7 polyclonal antibody (Cat # PAB27477) at 1-4 ug/mL dilutions shows positivity in vesicles. -
Gene Info — NSUN7
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Interactomes
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