RFK polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of RFK (arrow) using RFK polyclonal antibody (Cat # PAB2744).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RFK gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RFK polyclonal antibody (Cat # PAB2744) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RFK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RFK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of RFK (arrow) using RFK polyclonal antibody (Cat # PAB2744).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RFK gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RFK polyclonal antibody (Cat # PAB2744) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — RFK
Entrez GeneID
55312Protein Accession#
NP_060809;Q969G6Gene Name
RFK
Gene Alias
FLJ11149, RIFK
Gene Description
riboflavin kinase
Gene Ontology
HyperlinkGene Summary
Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM
Other Designations
0610038L10Rik|ATP:riboflavin 5'-phosphotransferase|flavokinase
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Interactome
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Pathway
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Publication Reference
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Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism.
Karthikeyan S, Zhou Q, Osterman AL, Zhang H.
Biochemistry 2003 Nov; 42(43):12532.
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Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch.
Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H.
Structure 2003 Mar; 11(3):265.
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Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism.
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