RC3H1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RC3H1.
Immunogen
A synthetic peptide corresponding to 18 amino acids near C-terminus of human RC3H1.
The immunogen is located within amino acids 1040-1090 of RC3H1.Host
Rabbit
Theoretical MW (kDa)
125
Reactivity
Human, Mouse, Rat
Specificity
At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (5 ug/mL)
Immunofluorescence (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for three months. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RC3H1 in HeLa cell lysate with RC3H1 polyclonal antibody (Cat # PAB27394) at 1 ug/mL.Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RC3H1
Entrez GeneID
149041Protein Accession#
NP_742068Gene Name
RC3H1
Gene Alias
KIAA2025, RNF198, ROQUIN
Gene Description
ring finger and CCCH-type zinc finger domains 1
Omim ID
609424Gene Ontology
HyperlinkGene Summary
RC3H1, or roquin, encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al., 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM
Other Designations
OTTHUMP00000060492|roquin
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