KRT8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT8.
Immunogen
A synthetic peptide corresponding to human KRT8.
Host
Rabbit
Theoretical MW (kDa)
52
Reactivity
Human, Mouse
Specificity
KRT8 polyclonal antibody detects endogenous levels of KRT8 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with Anisomycin 25ug/ml 30' treated. Using KRT8 polyclonal antibody (Cat # PAB27073).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using KRT8 polyclonal antibody (Cat # PAB27073).Immunofluorescence
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Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P05787Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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