HSP90B1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with HSP90B1 polyclonal antibody (Cat # PAB27069) at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using HSP90B1 polyclonal antibody (Cat # PAB27069).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSP90B1.
Immunogen
A synthetic peptide corresponding to amino acids 760-810 of human HSP90B1.
Host
Rabbit
Theoretical MW (kDa)
~95.0
Reactivity
Human, Mouse
Specificity
HSP90B1 polyclonal antibody detects endogenous levels of HSP90B1 protein.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with HSP90B1 polyclonal antibody (Cat # PAB27069) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using HSP90B1 polyclonal antibody (Cat # PAB27069).Immunofluorescence
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Gene Info — HSP90B1
Entrez GeneID
7184Protein Accession#
P14625Gene Name
HSP90B1
Gene Alias
ECGP, GP96, GRP94, TRA1
Gene Description
heat shock protein 90kDa beta (Grp94), member 1
Omim ID
191175Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
Tumor rejection antigen-1 (gp96)|endothelial cell (HBMEC) glycoprotein|glucose regulated protein, 94 kDa|heat shock protein 90kDa beta, member 1|tumor rejection antigen (gp96) 1
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Interactome
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