EGFR polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
Immunogen
A synthetic peptide corresponding to human EGFR.
Host
Rabbit
Theoretical MW (kDa)
175
Reactivity
Human, Mouse, Rat
Specificity
EGFR polyclonal antibody detects endogenous levels of EGFR protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EGFR polyclonal antibody (Cat # PAB27060) in extracts from 293 cells treated with EGF 200 ng/mL 30 mins.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of EGFR polyclonal antibody (Cat # PAB27060) in paraffin-embedded human breast carcinoma tissue.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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