E2F4/E2F5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of E2F4/E2F5.
Immunogen
A synthetic peptide corresponding to human E2F4/E2F5.
Host
Rabbit
Theoretical MW (kDa)
44
Reactivity
Human, Mouse, Rat
Specificity
E2F4/E2F5 polyclonal antibody detects endogenous levels of E2F4/E2F5 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of E2F4/E2F5 polyclonal antibody (Cat # PAB27055) in extracts from COS-7 cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of E2F4/E2F5 polyclonal antibody (Cat # PAB27055) in paraffin-embedded human lung carcinoma tissue.Enzyme-linked Immunoabsorbent Assay
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Gene Info — E2F4
Entrez GeneID
1874Protein Accession#
Q16254 (Gene ID : 1874);Q15329 (Gene : 1875)Gene Name
E2F4
Gene Alias
E2F-4
Gene Description
E2F transcription factor 4, p107/p130-binding
Omim ID
600659Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq
Other Designations
E2F transcription factor 4|p107/p130-binding protein
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Gene Info — E2F5
Entrez GeneID
1875Protein Accession#
Q16254 (Gene ID : 1874);Q15329 (Gene : 1875)Gene Name
E2F5
Gene Alias
E2F-5
Gene Description
E2F transcription factor 5, p130-binding
Omim ID
600967Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq
Other Designations
E2F transcription factor 5
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Interactome
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Pathway
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