CLDN3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CLDN3.
Immunogen
A synthetic peptide corresponding to human CLDN3.
Host
Rabbit
Theoretical MW (kDa)
25
Reactivity
Human, Mouse, Rat
Specificity
CLDN3 polyclonal antibody detects endogenous levels of CLDN3 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CLDN3 polyclonal antibody (Cat # PAB27035) in extracts from HUVEC.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of CLDN3 polyclonal antibody (Cat # PAB27035) in paraffin-embedded human colon carcinoma tissue.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLDN3
Entrez GeneID
1365Protein Accession#
O15551Gene Name
CLDN3
Gene Alias
C7orf1, CPE-R2, CPETR2, HRVP1, RVP1
Gene Description
claudin 3
Omim ID
602910Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq
Other Designations
CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|OTTHUMP00000025042|OTTHUMP00000160506|claudin-3|ventral prostate.1-like protein
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Interactome
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Pathway
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