PRKAA1/PRKAA2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAA1/PRKAA2.
Immunogen
A synthetic peptide corresponding to amino acids 150-200 of human PRKAA1.
Host
Rabbit
Theoretical MW (kDa)
63
Reactivity
Human, Mouse, Rat
Specificity
PRKAA1/PRKAA2 polyclonal antibody detects endogenous levels of PRKAA1/PRKAA2 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) Hela cell lysate, (2) mouse brain tissue lysate and (3) rat brain tissue lysate at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue at 1:100. -
Gene Info — PRKAA1
Entrez GeneID
5562Protein Accession#
Q13131 (Gene ID : 5562);P54646 (Gene ID : 5563)Gene Name
PRKAA1
Gene Alias
AMPK, AMPKa1, MGC33776, MGC57364
Gene Description
protein kinase, AMP-activated, alpha 1 catalytic subunit
Omim ID
602739Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-1 chain|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1
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Gene Info — PRKAA2
Entrez GeneID
5563Protein Accession#
Q13131 (Gene ID : 5562);P54646 (Gene ID : 5563)Gene Name
PRKAA2
Gene Alias
AMPK, AMPK2, PRKAA
Gene Description
protein kinase, AMP-activated, alpha 2 catalytic subunit
Omim ID
600497Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-2 chain|AMP-activated protein kinase alpha 2 catalytic subunit|AMPK-alpha-2 chain|OTTHUMP00000009993
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Interactome
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Pathway
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Disease
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