YWHAZ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of YWHAZ polyclonal antibody (Cat # PAB27012) in extracts from 293 cells treated with Forskolin 40 nM 30' and NIH/3T3 cells treated with PMA 250 ng/mL 5'.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of YWHAZ polyclonal antibody (Cat # PAB27012) in paraffin-embedded human breast carcinoma tissue.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YWHAZ.
Immunogen
A synthetic peptide corresponding to human YWHAZ.
Host
Rabbit
Theoretical MW (kDa)
28
Reactivity
Human, Mouse, Rat
Specificity
YWHAZ polyclonal antibody detects endogenous levels of YWHAZ protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitor (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of YWHAZ polyclonal antibody (Cat # PAB27012) in extracts from 293 cells treated with Forskolin 40 nM 30' and NIH/3T3 cells treated with PMA 250 ng/mL 5'.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of YWHAZ polyclonal antibody (Cat # PAB27012) in paraffin-embedded human breast carcinoma tissue.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAZ
Entrez GeneID
7534Protein Accession#
P63104Gene Name
YWHAZ
Gene Alias
KCIP-1, MGC111427, MGC126532, MGC138156
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Omim ID
601288Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein/cytosolic phospholipase A2|14-3-3 zeta|OTTHUMP00000165851|OTTHUMP00000165852|OTTHUMP00000165854|OTTHUMP00000165858|OTTHUMP00000165859|OTTHUMP00000165860|phospholipase A2|protein kinase C inhibitor protein-1|tyrosine 3/tryptophan 5 -monooxyg
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Interactome
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Pathway
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Disease
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