MARK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The MARK1 polyclonal antibody (Cat # PAB2698) is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 polyclonal antibody (Cat # PAB2698) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MARK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MARK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The MARK1 polyclonal antibody (Cat # PAB2698) is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 polyclonal antibody (Cat # PAB2698) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — MARK1
Entrez GeneID
4139Protein Accession#
NP_061120;Q2HIY1Gene Name
MARK1
Gene Alias
KIAA1477, MARK, MGC126512, MGC126513
Gene Description
MAP/microtubule affinity-regulating kinase 1
Omim ID
606511Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035458
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Interactome
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Disease
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Publication Reference
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Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms.
Hezel AF, Gurumurthy S, Granot Z, Swisa A, Chu GC, Bailey G, Dor Y, Bardeesy N, Depinho RA.
Molecular and Cellular Biology 2008 Jan; 28(7):2414.
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Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms.
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