EIF4G2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4G2.
Immunogen
A synthetic peptide corresponding to human EIF4G2.
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse
Specificity
EIF4G2 polyclonal antibody detects endogenous levels of EIF4G2 protein.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-549 cell lysate with EIF4G2 polyclonal antibody (Cat # PAB26903).Immunohistochemistry
Immunofluorescence
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Gene Info — EIF4G2
Entrez GeneID
1982Protein Accession#
P78344Gene Name
EIF4G2
Gene Alias
AAG1, DAP5, FLJ41344, NAT1, p97
Gene Description
eukaryotic translation initiation factor 4 gamma, 2
Omim ID
602325Gene Ontology
HyperlinkGene Summary
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq
Other Designations
aging-associated protein 1|death-associated protein 5|eIF-4-gamma 2|eukaryotic translation initiation factor 4G-like 1
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